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Chin. Opt. Lett.
 Home  List of Issues    Issue 06 , Vol. 07 , 2009    10.3788/COL20090706.0542

Identification of lactobacillus casei-BDI and Streptococcus thermophilus by fluorescence spectra
Hao Wu1, Tuo Zhu1, Wei Chen2, Hao Zhang2, Jianlei Yang3
1School of Science, [Jiangnan University], Wuxi 214122, China
2 State Key Laboratory of Food Science and Technology, School of Food Science and Technology, [Jiangnan University], Wuxi 214122, China

3 School of Communication and Control Engineering, [Jiangnan University], Wuxi 214122, China
E-mail: tzhu@jiangnan.edu.cn

Chin. Opt. Lett., 2009, 07(06): pp.542-544-3

Keywords(OCIS Code): 300.6540  300.6280  000.1430  

Fluorescence analysis applied in the study of lactic acid bacteria (LAB) provides a new method and theory to study probiotics and realize the detection and identification of the strains. It is also possible to achieve automation and computerization. In this letter, the differences between the fluorescence spectra of lactobacillus casei-BDI (Lc-BDI) and Streptococcus thermophilus (St) are shown, and the second-order derivative spectra are used to further study the diversity of these two strains. According to the results, with the excitation wavelengths of 285 and 340 nm, there are significant differences between them. The experiment is repeated for 6 times, showing good repetitiveness.

Copyright: © 2003-2012 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Note: This work was supported by the National "863" Program of China (No. 2007AA10Z353) and the State Key Laboratory of Food Science and Technology.


1. G. Zhang, (ed.) Lactic Acid Bacteria (in Chinese) (Chemical Industry Press, Beijing, 2007).

2. T. Cheng, J. Wang, and H. Zhang, China Dairy Industry (in Chinese) 36, (7) 4 (2008).

3. H. Li, X. Song, Y. Li, and S. Wei, Progress in Veterinary Medicine (in Chinese) 29, (5) 94 (2008).

4. K. Yang, “Study on kinetics and FT-IR rapid detection of Alicyclobacillus acidoterrestris originated from apple juice” (in Chinese) Master Thesis (Northwest A&F University, Yangling, 2007).

5. Y. Shi, G. Liu, J. Liu, and D. Song, Acta Opt. Sin. (in Chinese) 27, 129 (2007).

6. M. S. Ammor, J. Fluoresc. 17, 455 (2007).

7. H. E. Giana, L. Silveira, Jr., R. A. Zangaro, and M. T. T. Pacheco, J. Fluoresc. 13, 489 (2003).

8. H. Jin, B. Yan, and Y. Wang, Journal of Yanshan University (in Chinese) 29, 493 (2005).

9. J. Ding, J. Li, M. Wu, X. Si, and G. Zhao, J. Optoelectron. Laser (in Chinese) 15, 1007 (2004).

10. T. Huang, Y. He, Y. Xiao, W. Xue, and G. Zhou, Chin. Opt. Lett. 2, 72 (2004).

11. Z. Chen, Z. Tang, W. Wan, and X. Wang, Chinese J. Lasers (in Chinese) 32, 775 (2005).

12. A. Shi, T. Zhu, E. Gu, Y. Zhang, and Z. Liu, Acta Opt. Sin. (in Chinese) 28, 2237 (2008).

13. M. Chen, J. Wu, Y. Lü, and Q. Chen, Acta Opt. Sin. (in Chinese) 28, 578 (2008).

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